Why are cut fragments that result in sticky ends easier to join than blunt end fragments
Favorites|Homepage
Subscriptions | sitemap
HOME > > Why are cut fragments that result in sticky ends easier to join than blunt end fragments

Why are cut fragments that result in sticky ends easier to join than blunt end fragments

[From: ] [author: ] [Date: 12-06-04] [Hit: ]
i:76-The unpaired bases of the sticky ends are sticky because they will form hydrogen bonds with complementary bases.This ability to associate increases the likelihood of chains being together in the presence of the DNA ligase.......
If possible, attach a link of a diagram too.

-
Sticky ends have "overhangs" which means one strand is a little longer than the other. If you have another fragment you want to insert in the genome (or another piece of DNA), and you cut it so it has a matching overhang, its easy for them to bind together. If there is no overhang, there is no easy way for the two pieces to bind together.

This shows you both sticky and blunt end examples:

http://www.google.com/imgres?num=10&hl=en&biw=1519&bih=668&tbm=isch&tbnid=8zxzsODhlGP1DM:&imgrefurl=https://bio4ugroup2.wikispaces.com/Biotechnology&docid=GGKTuZganWiPqM&imgurl=https://bio4ugroup2.wikispaces.com/file/view/image.jpg/68352639/408x360/image.jpg&w=340&h=300&ei=7qzLT8P9HsT76gGEuOAc&zoom=1&iact=hc&vpx=584&vpy=151&dur=985&hovh=211&hovw=239&tx=116&ty=125&sig=107249578083472940412&sqi=2&page=1&tbnh=154&tbnw=175&start=0&ndsp=21&ved=1t:429,r:2,s:0,i:76

-
The unpaired bases of the sticky ends are "sticky" because they will form hydrogen bonds with complementary bases. This ability to associate increases the likelihood of chains being together in the presence of the DNA ligase.
1
keywords: that,are,than,fragments,sticky,blunt,Why,end,cut,ends,in,join,easier,to,result,Why are cut fragments that result in sticky ends easier to join than blunt end fragments
New
Hot
© 2008-2010 http://www.science-mathematics.com . Program by zplan cms. Theme by wukong .