Microbial growth question.

Can anyone give me 1 or 2 disadvantages for using the turbidity method when examining microbial cells using the spectrometer. Thanks a million!

Big one is that you can only find a total concentration of cells, rather than just viable ones (dead cells will remain in culture so your concentration reading will account for both dead and living).

Also, when cells become highly concentrated, the accuracy of spectrophotometry drops off at a certain threshold.