Best method to filter a chloroform solution
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Best method to filter a chloroform solution

[From: ] [author: ] [Date: 11-05-14] [Hit: ]
What is the best method to do this? Ive been looking at bottle top vacuum filters with a PTFE, PVDF, or regenerated cellulose membrane, but Ive noticed almost all these bottle tops are made of polypropylene, which I understand only has limited compatibility with chloroform.......
I'm trying to purify a lipid dissolved in chloroform. In case this is not clear, the chloroform is the solvent - I am not trying to filter out the chloroform. I am trying to filter out lipid aggregates above a certain size.

What is the best method to do this? I've been looking at bottle top vacuum filters with a PTFE, PVDF, or regenerated cellulose membrane, but I've noticed almost all these bottle tops are made of polypropylene, which I understand only has limited compatibility with chloroform. I obviously don't want to leech anything out into my solution.

I've also looked at syringe filters, but again, same case with the filter housing.

Are there specially made products for halogenated hydrocarbons solutions (eg. made with glass housing)? Any links to the big suppliers (eg. Sigma, VWR, Fisher) are also appreciated. Thanks!

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Do you mean polycarbonate? Polypropylene IS resistant to chloroform. You're only filtering, after all, not reacting or storing in it, so the exposure is what - 10 seconds? The flanges will likely last longer than your career as a grad student. There's no plasticizer in polypropylene, and evidently there is nothing but micelles and solvent in your samples, so what harm do you foresee? You can always filter a sample and run it through the HPLC to see what new organics have crept into the solvent. Or, if you're not wedded to chloroform, you can resuspend your micelles in a more polar solvent (though to my way of thinking that's a lot dicier than using chloroform in polypropylene).

Or, find a paper with a prep step like this one, and contact the author of that paper and ask for procedural details.

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